DDX4 antibody

Catalog No. ABIN965984

The Mouse Monoclonal anti-DDX4 antibody has been validated for WB, ELISA, IHC, FACS and IF. It is suitable to detect DDX4 in samples from Human. There are 3+ publications available.

Quick Overview for DDX4 antibody (ABIN965984)

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 2F9H5

Product Details anti-DDX4 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human DDX4 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-DDX4 antibody (ABIN965984)

Shibata, Tsunekawa, Okamoto-Ito, Akasu, Tokumasu, Noce: "Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein." in: Molecular reproduction and development, Vol. 67, Issue 1, pp. 1-7, (2003) (PubMed).

Jones: "Predicability (ease of predication) as semantic substrate of imageability in reading and retrieval." in: Brain and language, Vol. 82, Issue 2, pp. 159-66, (2002) (PubMed).

Castrillon, Quade, Wang, Quigley, Crum: "The human VASA gene is specifically expressed in the germ cell lineage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9585-90, (2000) (PubMed).

Target Details for DDX4

Target: DDX4 (DEAD Box Protein 4 (DDX4))

Alternative Name: DDX4

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Synonyms: VASA,MGC111074,DDX4

Molecular Weight: 76kDa

Gene ID: 54514